A diaphragm is the part of the microscope that makes an image brighter. When using a microscope, it is often for a scientific purpose so the image needs to be as clear as possible and part of this is ensuring that the brightness is at the right level.
If an image or slide is too dark then you run into the risk of miscounting or misrepresenting the data you have collected. To make an image brighter, you will need to adjust the diaphragm which can be located under the stage above the condenser. To learn more about microscopes and how to use them make sure to check out our section on this topic.
A camera is incorporated with a number of tools to manipulate the resulting image. If you want to make the image brighter, you need to make the aperture wider to allow more light to fall through. This will light up the image much more, increasing the brightness. To do this, the automatic setting on the camera manipulates the diaphragm within the camera, which in turn alter the width of the aperture.
This will make more light to pass through the camera lens, giving the pictures a greater brightness. Modern cameras come with automatic settings that you can easily use to alter different aspects according to your wishes and the brightness is one of them.