DNA Polymerase III is the primary enzyme involved in DNA replication. DNA Polymerase synthesizes base pairs after strand separation at the origin of replication. As the replication progresses, and the replisome moves forward, DNA Polymerase III arrives at RNA primer and begins replicating DNA. The lagging strand is the new strand that grows directly away from the replication fork. It is able to be replicated by continuously by DNA Polymerase.
DNA Polymerase only functions in the 5’ to 3’ direction. The enzyme that catalyzes the DNA strand elongation is DNA Polymerase III. It is also the vital enzymes for the prokaryotic replication.