Which is a template independent DNA polymerase?

Asked by Karthikeyan Pethusamy, Last updated: Oct 23, 2024

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3 Answers

J. Harty, Writer, M.A, Chula Vista

Answered Dec 26, 2018

Correct answer is Terminal deoxynucleotidyl transferase (TdT).
TdT is a template independent DNA polymerase. It does not require a template and it will not copy one. It catalyzes the incorporation of single deoxynucleotide into the 3’ - hydroxyl terminus (3’ – OH) of DNA which is accompanied by the release of inorganic phosphate. For this action, cobalt is a necessary cofactor.

Tdt is expressed in immature, pre B and T lymphoid cells and acute lymphoblastic lymphoma cells. It is expressed in lymphoid organs such as thymus and bone marrow.
Therefore, option A, B and D is not correct.

John Adney

Answered Nov 24, 2017

But PAP enzyme also not require template for ploy A polymerization

Karthikeyan Pethusamy, Research Associate, AIIMS, Delhi, MBBS MD DNB, Delhi

Answered Nov 25, 2017

Terminal deoxynucleotidyl transferase

Usually DNA & Polymerases need a template. But certain polymerases are unique and can add nucleotides without a template.